[Preliminarily study of arthrocentesis combined with liquid phase concentrated growth factor injection in the treatment of unilateral temporomandibular joint osteoarthritis].

OBJECTIVE: To observe the clinical effect of arthrocentesis combined with liquid phase concentrated growth factor (CGF) injection in the treatment of unilateral temporomandibular joint osteoarthritis (TMJOA), in order to provide a new treatment option for TMJOA patients. METHODS: In this non-randomized controlled study, patients diagnosed with unilateral TMJOA who visited the center for temporomandibular joint disorder and orofacial pain of Peking University School and Hospital of Stomatology from June 2021 to January 2023 were selected as research objects. The patients were divided into experimental group and control group, which were selected by patients themselves. The experimental group received arthrocentesis combined with liquid phase CGF injection and the control group received arthrocentesis combined with HA injection. Both groups were treated 3 times, once every two weeks. The clinical effect was evaluated by the maximum mouth opening, pain value and the degree of mandibular function limitation 6 months after treatment. The change of condylar bone was evaluated by cone beam CT (CBCT) image fusion technology before and after treatment. RESULTS: A total of 20 patients were included in the experimental group, including 3 males and 17 females, with an average age of (34.40±8.41) years. A total of 15 patients were included in the control group, including 1 male and 14 females, with an average age of (32.20±12.00) years. There was no statistical difference in general information between the two groups (P > 0.05). There were no statistical differences in the mouth opening, pain value and the degree of jaw function limitation between the two groups before treatment (P > 0.05), and all of them improved 6 months after treatment compared with before treatment (P < 0.05). However, the mouth opening of experimental group was significantly higher than that of control group 6 months after treatment (P < 0.05), and the degree of jaw function limitation was significantly lower than that of control group (P < 0.05). CBCT 2D images showed that the condylar bone of both groups was smoother after treatment than before treatment, and image fusion results showed that 10 patients (50.0%) in the experimental group and 5 patients (33.3%) in the control group had reparative remodeling area of condylar bone, and there was no statistical difference between them (P > 0.05). Except for one CGF patient, the other patients in both groups had some absorption areas of condylar bone. CONCLUSION: The arthrocentesis combined with liquid phase CGF injection can improve the clinical symptoms and signs of unilateral TMJOA patients in short term, and is better than HA in increasing mouth opening and improving jaw function. CBCT fusion images of both patient groups show some cases of condylar bone reparative remodeling and its relevance to treatment plans still requires further study.

A new strategy for improving the energy efficiency of electro-Fenton: Using N-doped activated carbon cathode with strong Fe(III) adsorption capacity to promote Fe(II) regeneration.

Fe(II) regeneration plays a crucial role in the electro-Fenton process, significantly influencing the rate of ·OH formation. In this study, a method is proposed to improve Fe(II) regeneration through N-doping aimed at enhancing the adsorption capacity of the activated carbon cathode for Fe(III). N-doping not only enriched the pore structure on the surface of activated carbon, providing numerous adsorption sites, but also significantly increased the adsorption energy for Fe(III). Among the types of nitrogen introduced, pyridine-N exhibited the most substantial enhancement effect, followed by pyrrole-N, while graphite-N showed a certain degree of inhibition. Furthermore, N-doping facilitated the adsorption of all forms of Fe(III) by activated carbon. The adsorption and electrosorption rates of the NAC-900 electrode for Fe(III) were 30.33% and 42.36%, respectively. Such modification markedly enhanced the Fe3+/Fe2+ cycle within the electro-Fenton system. The NAC-900 system demonstrated an impressive phenol degradation efficiency of 93.67%, alongside the lowest electricity consumption attributed to the effective "adsorption-reduction" synergy for Fe(III) on the NAC-900 electrode. Compared to the AC cathode electro-Fenton system, the degradation efficiency of the NAC-900 cathode electro-Fenton system at pH = levels ranging from 3 to 5 exceeded 90%; thus, extending the pH applicability of the electro-Fenton process. The degradation efficiency of phenol using the NAC-900 cathode electro-Fenton system in various water matrices approached 90%, indicating robust performance in real wastewater treatment scenarios. This research elucidates the impact of cathodic Fe(III) adsorption on Fe(II) regeneration within the electro-Fenton system, and clarifies the influence of different N- doping types on the cathodic adsorption of Fe(III).

Prospective role of lusianthridin in attenuating cadmium-induced functional and cellular damage in rat thyroid.

The thyroid represents the most prevalent form of head and neck and endocrine cancer. The present investigation demonstrates the anticancer effects of Lusianthridin against cadmium (Cd)-induced thyroid cancer in rats. Swiss Wistar rats were utilized in this experimental study. Cd was employed to induce thyroid cancer, and the rats were divided into different groups, receiving oral administration of Lusianthridin (20 mg/kg) for 14 days. Thyroid parameters, deiodinase levels, hepatic parameters, lipid parameters, and antioxidant parameters were respectively estimated. The mRNA expression was assessed using real-time reverse transcriptase polymerase chain reaction (RT-PCR). Lusianthridin significantly (P < 0.001) improved protein levels, T4, T3, free iodine in urine, and suppressed the level of TSH. Lusianthridin significantly (P < 0.001) enhanced the levels of FT3, FT4, and decreased the level of rT3. Lusianthridin significantly (P < 0.001) reduced the levels of D1, D2, D3, and enhanced the levels of hepatic parameters like AST, ALT. Lusianthridin remarkably (P < 0.001) altered the levels of lipid parameters such as LDL, total cholesterol, HDL, and triglycerides; antioxidant parameters viz., MDA, GSH, CAT, and SOD. Lusianthridin significantly altered the mRNA expression of Bcl-2, Bax, MEK1, ERK1, ERK2, p-eIf2α, GRP78, eIf2α, and GRP94. The results clearly state that Lusianthridin exhibits protective effects against thyroid cancer.

Metagenomic insights into the response of soil microbial communities to pathogenic Ralstonia solanacearum.

Understanding the response of soil microbial communities to pathogenic Ralstonia solanacearum is crucial for preventing bacterial wilt outbreaks. In this study, we investigated the soil physicochemical and microbial community to assess their impact on the pathogenic R.solanacearum through metagenomics. Our results revealed that certain archaeal taxa were the main contributors influencing the health of plants. Additionally, the presence of the pathogen showed a strong negative correlation with soil phosphorus levels, while soil phosphorus was significantly correlated with bacterial and archaeal communities. We found that the network of microbial interactions in healthy plant rhizosphere soils was more complex compared to diseased soils. The diseased soil network had more linkages, particularly related to the pathogen occurrence. Within the network, the family Comamonadaceae, specifically Ramlibacter_tataouinensis, was enriched in healthy samples and showed a significantly negative correlation with the pathogen. In terms of archaea, Halorubrum, Halorussus_halophilus (family: Halobacteriaceae), and Natronomonas_pharaonis (family: Haloarculaceae) were enriched in healthy plant rhizosphere soils and showed negative correlations with R.solanacearum. These findings suggested that the presence of these archaea may potentially reduce the occurrence of bacterial wilt disease. On the other hand, Halostagnicola_larseniia and Haloterrigena_sp._BND6 (family: Natrialbaceae) had higher relative abundance in diseased plants and exhibited significantly positive correlations with R.solanacearum, indicating their potential contribution to the pathogen's occurrence. Moreover, we explored the possibility of functional gene sharing among the correlating bacterial pairs within the Molecular Ecological Network. Our analysis revealed 468 entries of horizontal gene transfer (HGT) events, emphasizing the significance of HGT in shaping the adaptive traits of plant-associated bacteria, particularly in relation to host colonization and pathogenicity. Overall, this work revealed key factors, patterns and response mechanisms underlying the rhizosphere soil microbial populations. The findings offer valuable guidance for effectively controlling soil-borne bacterial diseases and developing sustainable agriculture practices.

Cooperative Cu/Pd-Catalyzed 1,5-Boroacylation of Cyclopropyl-Substituted Alkylidenecyclopropanes.

A Cu/Pd-cocatalyzed 1,5-boroacylation of cyclopropyl-substituted ACPs with B2pin2 and acid chlorides has been developed. Using cyclopropyl-substituted ACPs as the starting material, a broad range of 1,5-boroacylated products with multiple functional groups was prepared in good yields with excellent regio- and stereoselectively. Both aromatic and aliphatic acid chlorides were tolerated in this reaction.

Ruthenium-Catalyzed Carbonylation of α-Aminoaryl-Tethered Alkylidenecyclopropanes: Synthesis of Eight-Membered Benzolactams.

The synthesis of medium-sized lactams is a great challenge because of the unfavorable transannular interactions and entropic barriers in the transition state. We have developed a ruthenium-catalyzed carbonylation of α-aminoaryl-tethered alkylidenecyclopropanes (ACPs) that allows for the efficient preparation of valuable eight-membered benzolactams under ligand-free conditions. The amino group served a dual role of both directing group and nucleophile to facilitate the metallacycle formation and the carbonylation.

Kinetically Controlled Structural Modulation of the Self-Assembled Silver Nanoclusters.

Metal nanoclusters (NCs) with atomic precision are growing into a fascinating class of building blocks for supramolecular chemistry. What makes it more interesting is the enhanced optical properties of the ordered structures, including aggregation-induced emission (AIE). However, algorithm dictating the self-assembly of metal NCs in multicomponent environment remains largely unknown, and effective means to manipulate the self-assembly is still lacking, especially under kinetic control. Herein, nanofibers which contain sub-1 nm nanowires and exhibit circularly polarized phosphorescence (CPP) are obtained from crystallization-induced self-assembly (CISA) of water-soluble, negatively charged silver NCs (Ag9 -NCs) in the presence of glutamic acid (Glu). By the introduction of a positively-charged additive (choline chloride, CC), the structure of the nanowires is modulated and the lateral interaction between adjacent nanofibers is adjusted, leading to simultaneous improvement of the phosphorescence and chirality which finally enhances CPP. Importantly, changing the time at which CC is introduced altered the kinetic pathway of the CISA, which enables to effectively manipulate both the final structures of the self-assembled Ag9 -NCs and the output of the optical signals.

Self-Assembly of Atomically Precise Silver Nanoclusters in Crowded Colloids into Ultra-Long Ribbons with Tunable Supramolecular Chirality.

Atomically precise metal nanoclusters (NCs) emerge as fascinating synthons in self-assembled materials. The self-assembly of metal NCs are highly sensitive to the environment because they have an inorganic-organic hybridized structure and a relatively complicated conformation. Here, it is shown that when confined in crowded colloids, a water-soluble Ag9 -cored nanocluster (Ag9 -NC) can self- assemble into ultra-long (up to millimeters) and photoluminescent ribbons with high flexibility. The ribbon contains rectangularly organized columns of Ag9 -NCs and can undergo secondary self-assembly to form bundled and branched structures. Formation of ribbons is observed in all the tested colloids, including lyotropic liquid crystals and disordered, three-dimensional network. The high viscosity/elasticity of the crowded colloids weakens gravity-induced sedimentation of the ribbons, leading to the formation of an interesting class of inorganic-organic composite materials where the hard Ag-containing skeleton strengthens the soft matter. The simultaneously occurring symmetry breaking during the self-assembly of Ag9 -NCs gives uncontrolled supramolecular chirality, which can be tuned through the majority rule and soldier-and-sergeant rule by the introduction of chiral seeds. The regulated chirality and the intrinsic photoluminescence of the Ag9 -NCs ribbons impart the composite material circularly polarized luminescence, opening the door for a variety of potential applications.

Full-color-emitting fluids from carbon dots stabilized in nonconventionally fluorescent micelles.

Red-emitting carbon dots from pyrolysis of citric acid in formamide, which are intrinsically water-insoluble, were solubilized and stabilized by the fluorescent micelles formed by a nonionic silicone surfactant and an anionic surfactant, leading to the formation of full-color-emitting colloids with good biocompatibility and a variety of potential applications.

Strategies for promoting the degradation of phenol by electro-Fenton: Simultaneously promoting the generation and utilization of H2O2.

The use of the electro-Fenton process to continuously generate H2O2 and efficiently degrade organic pollutants is considered a promising technology. The ratio of generation of H2O2 is usually regarded as the critical step; however, how the H2O2 is utilized is also of particular importance. Herein, activated carbon was activated at different temperatures and used to explore the effect of nitrogen doping on the production and utilization of H2O2 in the electro-Fenton-based degradation of organic pollutants. The experimental results indicate that nitrogen-doped activated carbon simultaneously promotes the generation and utilization of H2O2, which is attributed to the regulation of the competition between phenol and O2 adsorption by the doped nitrogen. Nitrogen doping not only improves 2e-ORR selectivity but also aggregates phenol near the cathode to balance the concentrations of phenol and ·OH. Density functional theory (DFT) calculations further confirmed that pyrrole-N as a dopant promoted the adsorption of phenol, while pyridine-N was more favorable for O2 adsorption. The unique balance of nitrogen types possessed by modified activated carbon NAC-750 permits the efficient synergistic generation and utilization of H2O2 in a balanced manner during the degradation of phenol. This work provides a new direction for the rational nitrogen-doping modification of activated carbon for the electro-Fenton-based degradation of organic pollutants.

Material Evaluation and Dynamic Powder Deposition Modeling of PEEK/CF Composite for Laser Powder Bed Fusion Process.

Polymeric composites such as Poly-ether-ether-ketone (PEEK)/carbon fiber (CF) have been widely utilized due to outstanding performances such as high specific strength and specific modulus. The PEEK/CF components via powder bed fusion additive manufacturing usually show brittle fracture behaviors induced by their poor interfacial affinity and inner voids. These defects are strongly associated with powder packing quality upon deposition. The particle dynamic model has been widely employed to study the interactions of particle motions. Powder property, bulk material property, and interfacial features of composite powders are key factors in the particle dynamic model. In this work, an efficient and systematic material evaluation is developed for composite powders to investigate their deposition mechanism. The discrete element method is utilized to simulate the dynamic behaviors of PEEK/CF composite powders. The powder properties, bulk material properties, and interfacial features of powders are calibrated and justified by experimental measurement, numerical simulation, and design of experiments. The particle dynamic model can explain the powder flow behaviors and interactions. The experimental and simulation AOR results show a maximal deviation of 4.89%. It reveals that the addition of short CF particles can assist the flow of PEEK powders and improve the packing quality of the composite powders. The results show an experimental improvement of 31.3% and 55.2% for PEEK/CF_30wt% and PEEK/CF_50wt%, with a simulated improvement of 27.4% and 50.2% for corresponding composite powders.

Room Temperature Phosphorescence Carbon Dots: Preparations, Regulations, and Applications.

Room temperature phosphorescence (RTP) materials have drawn considerable attention by virtue of their outstanding features. Compared with organometallic complexes and pure organic compounds, carbon dots (CDs) have emerged as a new type of RTP materials, which show great advantages, such as moderate reaction condition, low toxicity, low cost, and tunable optical properties. In this review, the important progress made in RTP CDs is summarized, with an emphasis on the latest developments. The synthetic strategies of RTP CDs will be comprehensively summarized, followed by detailed introduction of their performance regulation and potential applications in anti-counterfeiting, information encryption, sensing, light-emitting diodes, and biomedicine. Finally, the remaining major challenges for RTP CDs are discussed and new opportunities in the future are proposed.

Biogeochemical behavior and pollution control of arsenic in mining areas: A review.

Arsenic (As) is one of the most toxic metalloids that possess many forms. As is constantly migrating from abandoned mining area to the surrounding environment in both oxidation and reducing conditions, threatening human health and ecological safety. The biogeochemical reaction of As included oxidation, reduction, methylation, and demethylation, which is closely associated with microbial metabolisms. The study of the geochemical behavior of arsenic in mining areas and the microbial remediation of arsenic pollution have great potential and are hot spots for the prevention and remediation of arsenic pollution. In this study, we review the distribution and migration of arsenic in the mining area, focus on the geochemical cycle of arsenic under the action of microorganisms, and summarize the factors influencing the biogeochemical cycle of arsenic, and strategies for arsenic pollution in mining areas are also discussed. Finally, the problems of the risk control strategies and the future development direction are prospected.

Clustering-enhanced, nonconventional photoluminescence from a silicone surfactant.

By using the intrinsic nonconventional photoluminescence (n-PL) of organo-siloxane and the synergetic effect of the surfactant mixture, we report strong n-PL from aqueous colloids containing a nonionic silicone surfactant mixed with a traditional anionic surfactant, with an unprecedently high fluorescence quantum yield of up to 85.58%.

Carbon Dot-Based Hydrogels: Preparations, Properties, and Applications.

Hydrogels have extremely high moisture content, which makes it very soft and excellently biocompatible. They have become an important soft material and have a wide range of applications in various fields such as biomedicine, bionic smart material, and electrochemistry. Carbon dot (CD)-based hydrogels are based on carbon dots (CDs) and auxiliary substances, forming a gel material with comprehensive properties of individual components. CDs embedding in hydrogels could not only solve their aggregation-caused quenching (ACQ) effect, but also manipulate the properties of hydrogels and even bring some novel properties, achieving a win-win situation. In this review, the preparation methods, formation mechanism, and properties of CD-based hydrogels, and their applications in biomedicine, sensing, adsorption, energy storage, and catalysis -are summarized. Finally, a brief discussion on future research directions of CD-based hydrogels will be given.

Self-Assembly of Cholesteryl Carbon Dots with Circularly Polarized Luminescence in Solution and Solvent-Free Phases.

Incorporating carbon dots (CDs) into chiral self-assemblies will endow the system with intriguing optoelectronic, catalytic, and chiroptical activities. Utilization of chiral substituents to rationally manipulate chiral self-assembly of the CDs, however, remains a major challenge. In this work, cholesteryl monoprotected ethylene diamine was used as a precursor to synthesize CDs with a cholesteryl periphery. The rigid, apolar, and chiral cholesteryl facilitates the polarity-sensitive self-assembly of CDs in organic solvents, showing circularly polarized luminescence (CPL) with dissymmetry g-factor at 10-3 grade. Temperature-variable characterizations suggested the formation of thermotropic liquid crystals within a wide temperature range driven by the interdigitation of cholesteryl segments, which further anchor the graphitic CD cores into tetragonal and cubic arrays. Self-assembly in a solvent-free state arouses sufficient chirality transfer and boosted the g-factors to 10-2 order of magnitude. This work unveils multiple and chiral self-assembly of CDs controlled by the cholesteryl substituents, exhibiting variable architectures and tunable CPL.

Silencing circ_0000644 inhibits papillary thyroid cancer cell malignancy by combining with miR-671-5p to release the inhibition on ANXA2.

BACKGROUND: Papillary thyroid cancer (PTC) is life-threatening due to its malignant progression. Considerable evidence demonstrates that circular RNA (circRNA) regulates PTC development. This study aims to explore the mechanism of circ_0000644 modulating PTC malignant progression. METHODS: The RNA levels of circ_0000644, microRNA-671-5p (miR-671-5p) and annexin A2 (ANXA2) were detected by quantitative real-time polymerase chain reaction. Western blot was performed to check protein expression. Cell proliferation and cell apoptosis were investigated by 5-ethynyl-29-deoxyuridine and flow cytometry. Angiogenic capacity, migration and invasion were analyzed by tube formation assay and transwell assay. The interaction between miR-671-5p and circ_0000644 or ANXA2 was identified by dual-luciferase reporter assay. Xenograft mouse model assay was performed to analyze the effect of circ_0000644 on tumor formation in vivo. RESULTS: Circ_0000644 and ANXA2 expression was significantly upregulated, while miR-671-5p was downregulated in PTC tissues and cells when compared with control groups. Circ_0000644 knockdown inhibited PTC cell proliferation, tube formation, migration, and invasion, but induced apoptosis in vitro. Moreover, circ_0000644 knockdown led to delayed tumorigenesis in vivo. In addition, circ_0000644 acted as a miR-671-5p sponge and mediated PTC cell tumor properties through miR-671-5p. ANXA2 was identified as a target gene of miR-671-5p, and its overexpression relieved miR-671-5p-induced effects in PTC cells. Furthermore, circ_0000644 depletion inhibited ANXA2 production by combining with miR-671-5p. CONCLUSION: Circ_0000644 depletion repressed PTC cell tumor properties through the miR-671-5p/ANXA2 axis.

An attention transfer entropy based causality analysis with applications in rooting short-term disturbances for chemical processes.

The transfer entropy (TE) based causality analysis is able to provide a typical solution for fault rooting of industrial processes. However, short-term disturbances that occur during nominal operations of chemical processes are usually neglected because of the fixed time window of TE for global data distributions. Inspired by the selective attention idea, we propose attention transfer entropy (ATE) that helps to locate prominent targets. Concerning temporal features of industrial time series, prior knowledge is employed for constructing an interpretable model. We verify the reliability and effectiveness of the method with coal gasification process data. Additionally, the algorithm is compared to conventional causality analysis methods, proving that ATE enjoys excellent performances in rooting short-term disturbances with lower calculation burden.

Supramolecular structures from structurally persistent and surface active carbon dots in water.

Carbon dots (CDs) have developed into an important class of nanomaterials that have attracted increasing attention during the past decades. Despite numerous types of CDs reported to date, research on their self-assembly is still limited. Herein, we report for the first time the self-assembly of CDs in water, which show concentration-dependent aggregation behavior. The CDs used have a structural motif of a fully carbonized core surrounded by a highly condensed, polymeric network, to which triethylene glycol monomethyl ether (TGME) chains are grafted. When dissolved in water, they show a low critical aggregation concentration (cac) of 0.07 mg mL-1 with the lowest surface tension of ∼37 mN m-1. Above this cac, nanoclusters and vesicles are observed at relatively low and high concentrations, respectively. At an intermediate concentration, polymorphism is noticed where nanotubes coexist with nanorods. At an elevated temperature, the CDs become more hydrophobic due to the dehydration of peripheral TGME, which decreases the cac and triggers phase transfer from water to toluene. These surface active CDs were used to disperse and stabilize multi-walled carbon nanotubes in water, which showed much better performance than that of both traditional ionic and nonionic surfactants. Our work indicates that with a careful structural design, CDs can be developed into a new type of amphiphiles with properties superior to those of traditional surfactants in specific aspects.

Evolution trend of soil fertility in tobacco-planting area of Chenzhou, Hunan Province, China.

In this study, the data of fertility indicators of soil samples (0-20 cm) in 1980s, 2000 and 2015 in Chenzhou city were used, and the soil integrated fertility index (IFI) was calculated. The results showed that the soil pH was decreased, total nitrogen (TN), organic matter (OM), available phosphorus (AP) and potassium (AK), exchangeable calcium (Ca2+), magnesium (Mg2+) and available copper (Cu) contents were increased, total phosphorus (TP), available sulfur (S) and water-soluble chlorine (Cl-) contents were decreased, total potassium (TK), available boron (B), iron (Fe), manganese (Mn) and zinc (Zn) were decreased first and then increased. In 2015, most of the fields were higher in pH, OM, TN, AN, AK, Ca2+, Mg2+, S, Fe, Mn, Cu and Zn, suitable in B, but lower in TP, AP, TK, available molybdenum (Mo) and Cl-. Most of the fields were in the middle grade of IFI in 2000 and 2015, and the mean IFI increased from 0.492 to 0.556 from 2000 to 2015. Thus, for soil improvement, more attention should be paid to adjust soil pH, reduce the application of organic, nitrogen and calcium fertilizers, while increase the fertilizer application of other nutrients.